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Some of Our Favorite Publications

Recent (Past Five Years)

BRCA1/BARD1 is a nucleosome reader and writer.

Witus SR, Zhao W, Brzovic PS, Klevit RE. TRENDS BIOCHEM SCI. (2022).

Cullin-independent recognition of HHARI substrates by a dynamic RBR catalytic domain.

Reiter KH, Zelter A, Janowska MJ, Riffle M, Shulman N, MacLean BX, Tamura K, Chambers MC, MacCoss MJ, Davis TN, Guttman M, Brzovic PS, Klevit RE. STRUCTURE. (2022).

BRCA1/BARD1 site-specific ubiquitylation of nucleosomal H2A is directed by BARD1.

Witus SR, Burrell AL, Farrell DP, Kang J, Wang M, Hansen JM, Pravat A, Tuttle LM, Stewart MD, Brzovic PS, Chatterjee C, Zhao W, DiMaio F, Kollman JM, Klevit RE. NAT STRUC MOL BIO. (2021).

The BRCA1/BARD1 ubiquitin ligase and its substrates.

Witus SR, Stewart MD, Klevit RE. BIOCHEM J. (2021).

Mediator subunit Med15 dictates the conserved "fuzzy" binding mechanism of yeast transcription activators Gal4 and Gcn4.

Tuttle LM, Pacheco D, Warfield L, Wilburn DB, Hahn S, Klevit RE. NAT COM. (2021).

Toggle switch residues control allosteric transitions in bacterial adhesins by participating in a concerted repacking of the protein core.

Kisiela DI, Magala P, Interlandi G, Carlucci LA, Ramos A, Tchesnokova V, Basanta B, Yarov-Yarovoy V, Avagyan H, Hovhannisyan A, Thomas WE, Stenkamp RE, Klevit RE, Sokurenko EV. PLOS PATHOGENS. (2021).

Legionella effector MavC targets the Ube2N~Ub conjugate for noncanonical ubiquitination.

Puvar K, Iyer S, Fu J, Kenny S, Negrón Terón KI, Luo ZQ, Brzovic PS, Klevit RE, Das C. NAT COM. (2020).

Release of a disordered domain enhances HspB1 chaperone activity toward tau.

Baughman HER, Pham T-HT, Adams CS, Nath A, and Klevit RE. PNAS USA. (2020).

Who with whom: functional coordination of E2 enzymes by RING E3 ligases during poly-ubiquitylation.

Lips C, Ritterhoff T, Weber A, Janowska MK, Mustroph M, Sommer T, Klevit RE. EMBO J. (2020).

UbcH5 Interacts with Substrates to Participate in Lysine Selection with the E3 Ubiquitin Ligase CHIP.

Kanack A, Vittal V, Haver H, Keppel T, Gundry RL, Klevit RE, Scaglione KM. BIOCHEM. (2020).

Peeking from behind the veil of enigma: emerging insights on small heat shock protein structure and function.


Interplay of disordered and ordered regions of a human small heat shock protein yields an ensemble of 'quasi-ordered' states.

Clouser AF, Baughman HE, Basanta B, Guttman M, Nath A, Klevit RE. ELIFE. (2019).

BARD1 is necessary for ubiquitylation of nucleosomal histone H2A and for transcriptional regulation of estrogen metabolism genes.

Stewart MD, Zelin E, Dhall A, Walsh T, Upadhyay E, Corn JE, Chatterjee C, King MC, Klevit RE. PNAS USA. (2018).


Zinc-Dependent Structure of a Single Finger Domain of Yeast ADR1.

Parraga GE, Horvath S, Eisen A, Taylor WE, Hood L, Young ET, Klevit RE. SCIENCE. (1988).

Structure of a BRCA1-BARD1 heterodimeric RING-RING complex.

Brzovic P, Rajagopal P, Hoyt DW, King MC, Klevit RE. NATURE STRUC BIOL. (2001).

A UbcH5/Ub Noncovalent Complex is Required for BRCA1-mediated Ubiquitination.

Brzovic PS, Lissounov A, Christensen DE, Klevit, RE. MOL CELL. (2006).

Estrogen receptor alpha is a putative substrate for the BRCA1 ubiquitin ligase.

Eakin CM, Maccoss MJ, Finney GL, Klevit RE. PNAS USA. (2007).

E2:BRCA1 RING interactions dictate the synthesis of either mono- or specific poly-ubiquitin chain linkages.

Christensen DE, Brzovic PS, Klevit RE. NATURE STRUC MOL BIOL. (2007).

Ubiquitin in Motion: Structural studies of the E2~Ub conjugate.

Pruneda JN, Stoll KE, Bolton LJ, Brzovic PS, Klevit RE. BIOCHEMISTRY. (2011).

The N-terminal domain of αB-crystallin provides a conformational switch for multimerization and structural heterogeneity.

Jehle S, Vollmar B, Bardiaux B, Dove KK, Rajagopal P, #Gonen T, #Oschkinat H, and #Klevit RE. PNAS USA. (2011).

The acidic transcription activator Gcn4 binds the Mediator subunit Gal11/Med15 using a simple protein interface forming a fuzzy complex.

Brzovic PS, Heikaus CC, Kisselev L, Vernon R, Herbig E, Pacheco D, Warfield L, Littlefield P, Baker D, Klevit RE, and Hahn S. MOL CELL. (2011).

UbcH7 reactivity profile reveals Parkin and HHARI to be RING/HECT hybrids.

Wenzel DM, Lissounov, A, Brzovic, PS, Klevit, RE. NATURE. (2011).

Structure of an E3:E2~Ub complex reveals an allosteric mechanism shared among RING/U-box ligases.

Pruneda JN, Littlefield PJ, Soss SE, Nordquist KA, Chazin WJ, Brzovic PS, Klevit RE. MOL CELL (2012).

Activity-enhancing mutations in an E3 ubiquitin ligase identified by high-throughput mutagenesis.

Starita LM, Pruneda JN, Lo RS, Fowler DM, Kim HJ, Hiatt JB, Shendure J, Brzovic PS, Fields S, Klevit RE. PNAS USA. (2013).

Allosteric activation of the RNF146 ubiquitin ligase by a poly(ADP-ribosyl)ation signal.

DaRosa PA, Wang Z, Jiang X, Pruneda JN, Cong F, Klevit RE, Xu W. NATURE. (2015).

Intrinsic disorder drives N-terminal ubiquitination by Ube2W.

Vittal V, Shi L, Wenzel DM, Scaglione KM, Duncan ED, Basrur V, Elenitoba-Johnson KS, Baker D, Paulson HL, Brzovic PS, Klevit RE. NAT CHEM BIOL. (2015).

A conserved histidine modulates HSPB5 structure to trigger chaperone activity in response to stress-related acidosis.

Rajagopal P, Tse E, Borst AJ, Delbecq SP, Shi L, Southworth DR, Klevit RE. ELIFE. (2015).

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