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Some of Our Favorite Publications

Recent (Past Five Years)

Disordered region encodes α-crystallin chaperone activity toward lens client γD-crystallin.

Woods CN, Ulmer LD, Guttman M, Bush MF, Klevit RE. PNAS USA. (2023).

BRCA1/BARD1 intrinsically disordered regions facilitate chromatin recruitment and ubiquitylation.

Witus SR, Tuttle LM, Li W, Zelter A, Wang M, Kermoade KE, Wilburn DB, Davis TN, Brzovic PS, Zhao W, Klevit RE. EMBO J. (2023).

Cullin-independent recognition of HHARI substrates by a dynamic RBR catalytic domain.

Reiter KH, Zelter A, Janowska MJ, Riffle M, Shulman N, MacLean BX, Tamura K, Chambers MC, MacCoss MJ, Davis TN, Guttman M, Brzovic PS, Klevit RE. STRUCTURE. (2022).

BRCA1/BARD1 is a nucleosome reader and writer.

Witus SR, Zhao W, Brzovic PS, Klevit RE. TRENDS BIOCHEM SCI. (2022).

Toggle switch residues control allosteric transitions in bacterial adhesins by participating in a concerted repacking of the protein core.

Kisiela DI, Magala P, Interlandi G, Carlucci LA, Ramos A, Tchesnokova V, Basanta B, Yarov-Yarovoy V, Avagyan H, Hovhannisyan A, Thomas WE, Stenkamp RE, Klevit RE, Sokurenko EV. PLOS PATHOGENS. (2021).

Mediator subunit Med15 dictates the conserved "fuzzy" binding mechanism of yeast transcription activators Gal4 and Gcn4.

Tuttle LM, Pacheco D, Warfield L, Wilburn DB, Hahn S, Klevit RE. NAT COM. (2021).

The BRCA1/BARD1 ubiquitin ligase and its substrates.

Witus SR, Stewart MD, Klevit RE. BIOCHEM J. (2021).

BRCA1/BARD1 site-specific ubiquitylation of nucleosomal H2A is directed by BARD1.

Witus SR, Burrell AL, Farrell DP, Kang J, Wang M, Hansen JM, Pravat A, Tuttle LM, Stewart MD, Brzovic PS, Chatterjee C, Zhao W, DiMaio F, Kollman JM, Klevit RE. NAT STRUC MOL BIO. (2021).

Peeking from behind the veil of enigma: emerging insights on small heat shock protein structure and function.


UbcH5 Interacts with Substrates to Participate in Lysine Selection with the E3 Ubiquitin Ligase CHIP.

Kanack A, Vittal V, Haver H, Keppel T, Gundry RL, Klevit RE, Scaglione KM. BIOCHEM. (2020).

Who with whom: functional coordination of E2 enzymes by RING E3 ligases during poly-ubiquitylation.

Lips C, Ritterhoff T, Weber A, Janowska MK, Mustroph M, Sommer T, Klevit RE. EMBO J. (2020).

Release of a disordered domain enhances HspB1 chaperone activity toward tau.

Baughman HER, Pham T-HT, Adams CS, Nath A, and Klevit RE. PNAS USA. (2020).

Legionella effector MavC targets the Ube2N~Ub conjugate for noncanonical ubiquitination.

Puvar K, Iyer S, Fu J, Kenny S, Negrón Terón KI, Luo ZQ, Brzovic PS, Klevit RE, Das C. NAT COM. (2020).


Structural Studies of HHARI/UbcH7~Ub reveal a unique mechanism of E2~Ub conformational restriction by RBR RING1.

Dove KK, Olszewski JL, Martino L, Duda DM, Wu XS, Miller DJ, Reiter KH, Rittinger K, Schulman BA, Klevit RE. STRUCTURE. (2017).

Two functionally distinct E2/E3 pairs coordinate sequential ubiquitination of a common substrate in Caenorhabditis elegans development.

Dove KK, Kemp HA, Di Bona KR, Reiter KH, Milburn LJ, Camacho D, Fay DS, Miller DL, Klevit RE. PNAS USA. (2017).

A conserved histidine modulates HSPB5 structure to trigger chaperone activity in response to stress-related acidosis.

Rajagopal P, Tse E, Borst AJ, Delbecq SP, Shi L, Southworth DR, Klevit RE. ELIFE. (2015).

Intrinsic disorder drives N-terminal ubiquitination by Ube2W.

Vittal V, Shi L, Wenzel DM, Scaglione KM, Duncan ED, Basrur V, Elenitoba-Johnson KS, Baker D, Paulson HL, Brzovic PS, Klevit RE. NAT CHEM BIOL. (2015).

Allosteric activation of the RNF146 ubiquitin ligase by a poly(ADP-ribosyl)ation signal.

DaRosa PA, Wang Z, Jiang X, Pruneda JN, Cong F, Klevit RE, Xu W. NATURE. (2015).

Activity-enhancing mutations in an E3 ubiquitin ligase identified by high-throughput mutagenesis.

Starita LM, Pruneda JN, Lo RS, Fowler DM, Kim HJ, Hiatt JB, Shendure J, Brzovic PS, Fields S, Klevit RE. PNAS USA. (2013).

Structure of an E3:E2~Ub complex reveals an allosteric mechanism shared among RING/U-box ligases.

Pruneda JN, Littlefield PJ, Soss SE, Nordquist KA, Chazin WJ, Brzovic PS, Klevit RE. MOL CELL (2012).

UbcH7 reactivity profile reveals Parkin and HHARI to be RING/HECT hybrids.

Wenzel DM, Lissounov, A, Brzovic, PS, Klevit, RE. NATURE. (2011).

The acidic transcription activator Gcn4 binds the Mediator subunit Gal11/Med15 using a simple protein interface forming a fuzzy complex.

Brzovic PS, Heikaus CC, Kisselev L, Vernon R, Herbig E, Pacheco D, Warfield L, Littlefield P, Baker D, Klevit RE, and Hahn S. MOL CELL. (2011).

The N-terminal domain of αB-crystallin provides a conformational switch for multimerization and structural heterogeneity.

Jehle S, Vollmar B, Bardiaux B, Dove KK, Rajagopal P, #Gonen T, #Oschkinat H, and #Klevit RE. PNAS USA. (2011).

Ubiquitin in Motion: Structural studies of the E2~Ub conjugate.

Pruneda JN, Stoll KE, Bolton LJ, Brzovic PS, Klevit RE. BIOCHEMISTRY. (2011).

E2:BRCA1 RING interactions dictate the synthesis of either mono- or specific poly-ubiquitin chain linkages.

Christensen DE, Brzovic PS, Klevit RE. NATURE STRUC MOL BIOL. (2007).

Estrogen receptor alpha is a putative substrate for the BRCA1 ubiquitin ligase.

Eakin CM, Maccoss MJ, Finney GL, Klevit RE. PNAS USA. (2007).

A UbcH5/Ub Noncovalent Complex is Required for BRCA1-mediated Ubiquitination.

Brzovic PS, Lissounov A, Christensen DE, Klevit, RE. MOL CELL. (2006).

Structure of a BRCA1-BARD1 heterodimeric RING-RING complex.

Brzovic P, Rajagopal P, Hoyt DW, King MC, Klevit RE. NATURE STRUC BIOL. (2001).

Zinc-Dependent Structure of a Single Finger Domain of Yeast ADR1.

Parraga GE, Horvath S, Eisen A, Taylor WE, Hood L, Young ET, Klevit RE. SCIENCE. (1988).

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